Chaetotaxy maps a well established tool for taxonomic relevance mainly in ticks and mites. Silver staining based tools have been employed in monogenean studies as well. Present communication deal with the application of silver staining technique for establishing argentophilic map of a Monogenean Mizelleus indicus (Jain, 1957), Pandey et al. 2003. Possibility of employing this tools in species validation is discussed in detail.