Objective: To determine the prevalence of Hepatitis C in patients undergoing hemodialysis in India. Materials and Methods: The study was a Prospective single centre observational study conducted in patients attending a Hemodialysis unit between April 2018 and September 2018 at Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar. All patients with Chronic Kidney Disease who underwent hemodialysis were included whereas patients with Acute Kidney Injury were excluded. All the patients were tested for anti-HCV antibodies using ELISA. Results: 100 patients out of which 62 male and 38 female were included. Overall mean age was 52 ± 8 years. Prevalence of Hepatitis C was found to be 38% among 100 patients undergoing Hemodialysis.