The present study was aimed to evaluate the modulatory role of L-ascorbic acid against oxidative stress in bifenthrin intoxicated rats. Rats were divided into four groups with six rats in each group. Group I animals received corn oil and served as control while as group II animals were orally treated with bifenthrin @ 5.8mg/Kg/day. In group III, vitamin C was orally administered @ 60mg/Kg/day where as group IV received both vitamin C and bifenthrin @ 60mg/Kg/day and 5.8mg/Kg/day respectively. After 30th day of treatment, heart samples were taken and analysed for oxidative stress parameters. Significant (P<0.05) increase in MDA levels was observed in bifenthrin treated animals as compared to control and vitamin C treated animals. The activities of antioxidant enzymes viz., SOD, GST and CAT decreased significantly (P<0.05) in bifenthrin treated rats as compared control. No significant change was observed in the concentration GSH-Px in bifenthrin intoxicated animals. Ameliorative group receiving both bifenthrin and L-ascorbic acid significantly restored the normal values of various altered oxidative stress parameters except catalase activity (CAT).