Background: Mycobacterium ulcerans infection often leads to extensive destruction of the skin and soft tissues, with extensive ulcerations usually occurring on the limbs. Confirmation of cases using gene amplification (PCR) or direct examination of smears has become an essential aspect in the overall management of the disease. Objective: To evaluate the sodium acetate method in the extraction of Mycobacterium ulcerans DNA for detection at PCR to confirm cases. Methods: Samples were taken from patients suspected of Buruli ulcer during the study period between January and April 2018 in selected sites. Analyzes were performed at the Mycobacteria laboratory using the Ziehl test, PCR by sodium acetate method and that using the Maxwell Kit. The results of the first two tests were compared to those of PCR by the Maxwell method used as a gold standard. The significance level was set at 5% and the 95% confidence interval (CI). Results: The proportion of positive samples was 16.5% for Ziehl, 21.7% for sodium acetate PCR and 35% for that using the Maxwell kit. PCR by the sodium acetate method yields sensitivity, specificity and VPP respectively of 52.9%, 95.2%, 85.7%. In contrast, the sensitivity, specificity and VPP for the Ziehl technique were 47.1%, 100% and 100%. Conclusion: Although the sensitivity is low, sodium acetate method can be used in absence of Maxwell Kit for extraction of Mycobacterium ulcerans DNA for detection and for confirmation of cases. It is the same for the Ziehl being a quick and simple test to perform in the case of detection.