Pests, microorganisms and parasites are responsible each year of substantial losses of crops. Among these crops, the highly perishable fruit must be harvested with care to avoid shock or injuries causing senescence and their alterations by pathogenic microorganisms. The pineapple (Ananas comosus. (L) M) is the third most produced tropical fruit after bananas and lemon. Ivory Coast, the first fresh pineapple supplier of the European Union with a coverage rate of 97% is facing several problems including the action of microorganisms during conservation and storage. Indeed, the pineapple with its biochemical composition is subject to the action of fungal strains responsible alterations and that may damage the health of humans. The use of chemical substances during conservation and storage although having solved the problem presents disadvantages vis-à-vis consumers. They are increasingly demanding and advocates organically produced "healthy". It would therefore be necessary to develop substances and biological organisms to not only protect the fruit but also the health of the consumer. Which will deal with post harvest losses and the harmful effects of chemical pesticides.