Objective: The main objective of the study is to formulate polyherbal formulation which is more effective at a lesser dose. Methods: In our present study, three plants were extracted and polyherbal formulation was prepared The CMC suspension of Polyherbal formulation was investigated for its anti ulcer activity against ethanol induced ulcer in rats at 100 and 200 mg/kg body weight p.o. Omeprazole was used as standard at a dose of 20 mg/kg. Five groups of adult albino rats were orally pre-treated respectively with CMC solution (ulcer positive control group),Group pre treated with CMC suspension(ulcer negative control group), Omeprazole 20 mg/kg (standard group), 100 mg/kg of Polyherbal formulation(PHF) suspended in CMC suspension(experimental group-1),and 200mg/kg of polyherbal formulation Suspended in CMC suspension(experimental group-2) one hour before oral administration of absolute ethanol to produce gastric mucosal injury. Rats were sacrificed and the ulcer areas of the gastric walls were determined. Results: Pretreatment of rats with PHF suspension produced protection in the ethanol induced ulceration model as compared to control group. The protection was statistically significant and reduced the severity of ulcer and caused a significant reduction of ulcer index in this model. Conclusion: The present study indicates that Polyherbal formulation have potential antiulcer activity in the model tested.