Statement of problem: Adherence and candidal colonization on resilient denture liner materials plays an important role in the pathogenesis of denturestomatitis. It has been reported that combining anti-fungal agents into these denture relining materials might be used in the treatment and prevention of denture stomatitis. Purpose of study: The aim of the study was to compare and evaluate the effectiveness of incorporation of two antifungal agents- Clotrimazole (C) andFluconazole (F) into the two auto-polymerized acrylic based resilient denture liners (Permasoft & Soft-liner) on the growth and colonization of candida albicans and to suggest a better antifungal agent out of them used in the study. Materials and Methods: Acrylic based resilient denture liners Permasoft and Soft-liner were incorporated with Clotrimazole (C) and Fluconazole (F)drugs both at 1% wt/wt concentration and experimental circular disc shaped specimen were fabricated using brass die with cross-section (20x4) mm. For fungal growth assessment, they were inoculated with C.albicans suspension prepared according to Clinical Laboratory and Standards Institute (CLSI) guidelines. The discs were incubated at 370 C and after incubation they were rinsed and centrifuged in sterile water to remove surface organisms. The drug treated and control circular disc shaped specimens were stored in distilled water for 30, 60 and 90 days and were wiped daily with wet cotton for 1 minute. Assessment of colony forming units (CFU) was carried out using McFarland turbidity standards as per CLSI guidelines. The data were compared using One-way analysis of variance (ANOVA), Repeated measure analysis of variance (ANOVA) and Paired ‘t’ Test. Results: The mean colony forming units CFU(x106/ml) at different time intervals differ significantly among groups; (Permasoft & Soft-liner) linermaterial (p<0.05). The values also differ highly significantly among subgroups; (Control, Clotrimazole and Fluconazole) and with both groups & subgroups (p<0.001). Conclusion: The addition of Clotrimazole and Fluconazole into acrylic based resilient denture liners Permasoft and Soft-liner significantly reduced thegrowth and colonization of C.albicans. The decrease in the mean colony forming units (CFUx106/ml) after 30 days followed by increase in them after 60 and 90 days interval was observed as compared to that of control (p<0.001). Combination of SOFT-LINER with Clotrimazole is found to be more effective than PERMASOFT with Clotrimazole and combination of PERMASOFT with Fluconazole is found to be more effective than SOFT-LINER with Fluconazole after subsequent time intervals.