The antibacterial action of honey on five bacterial clinical isolates (Staphylococcus aureus, E.coli, Pseudomonas aeruginosa, Klebsiella sp and Proteus sp) was observed with 50% (v/v) as well as the neat concentration (100%) v/v, using agar- ditch diffusion technique. All the tested organisms were resistant to concentration of honey at 6.5, 12.5 and 25% (v/v).E.coli showed the highest zone of inhibition which increases with increased in concentration followed by Klebsiella sp, Proteus sp and Staphylococcus aureus. While Pseudomonas aruginosa showed the least. The minimum inhibitory concentration (MIC) of honey presented Escherichia coli as the most susceptible organism and Pseudomonas aeruginosa also, the least.